Hi
Presumably you are talking about pix/asa device.
If you just need to allow .49 then just allow that host only in the access-list ie.
access-list outbound permit tcp host 10.220.4.49 any eq 80
etc...
However if you would like to allow all the 10.220.4.0/24 network other than IP addresses 1 -> 48 which i think is what you are asking
LabProtect1(config)# object-group network test
LabProtect1(config-network)# network-object 10.220.4.0 255.255.255.224
LabProtect1(config-network)# network-object 10.220.4.32 255.255.255.240
LabProtect1(config)# access-list outbound deny ip object-group TEST any
LabProtect1(config)# access-list outbound permit ip 10.224.4.0 255.255.255.0 any
HTH
Jon