I have configured intervlan in Multilayer switches. But i need to separate one vlan, vlan 100 from other vlan..but i just allow only subnet on vlan 100 can access the vlan 100..all other subnet in other vlan cannot access it. How the configuration works?
Yes,u said right regarding gateway.
VACL can be used for controlling traffic within a VLAN as well as traffic flowing in and out of a vlan
For configuration refer