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error dot1x autherdication 24427 Access to Active Directory failed

Hi

We are using ACS 5.0 and enabled dot1x , the access switch are configured dot1x port based auth , we are getting error 24427 Access to Active Directory failed ,

the isssue could be the ACS is not talking to AD , but the test connection was sucessfull , pl let me know what are all the ports need to be open for this

already opened ports 3268,464,123,389,445,88,135,137,139.

regards

krishna

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