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Cisco ACS 5.3 error 24401 Could not establish connection with ACS Active Directory agent : Authentication failed

We are implementing PEAP authentication with Active Directory using RADIUS

We join ACS  to the domain and it's status is Connected. I also import two group from AD.

But When I trying to authenticate users it give me below error

24401 Could not establish connection with ACS Active Directory agent :                                                        Authentication failed

I have installed lattest patch for ACS 5.3

When we create local users on ACS it authenticate sucessfully but when I use AD authentication it give me error.

I appriciate if any one have suggestion for this problem

Thank you,

Nilay

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